Extended Data Fig. 10: VMHvl c-Fos quantification controls.
From: A circuit from hippocampal CA2 to lateral septum disinhibits social aggression
a, Representative images of c-Fos immunofluorescence in VMH following bouts of aggression 30 min after intraperitoneal injection of CNO or saline in wild-type or Amigo2-Cre mice (all groups previously injected in CA2 with rAAV2-hsyn-DIO-HA-hM4D(Gi)-IRES-mCitrine). Scale bars, 400 µm. b, Number of c-Fos-expressing cells in VMHvl of wild-type mice killed 1 h after indicated behaviours. Coloured dots, individual mice (5, 6 and 9 mice). Bars show mean ± s.e.m. Kruskal–Wallis test χ2 = 15.16, P < 0.0001 followed by Dunn’s multiple comparison tests, ***P = 0.0006 and *P = 0.01. c, Each mouse was challenged with a resident–intruder test once every 5 days until it showed aggression, after which it was killed. Grey dots, individual mice (7, 8, 7 and 13). Bars show mean ± s.e.m. Two-way ANOVA: F = 7.4, *P = 0.01.
