Extended Data Fig. 1: CA2 projections to LS.
From: A circuit from hippocampal CA2 to lateral septum disinhibits social aggression
a–c, Diagram and horizontal sections from an Amigo2-Cre mouse brain injected with rAAV5-EF1a-DIO-hChR2(E123T/T159C)-eYFP into dCA2. The arrowhead in b shows the dCA2 axons extending up to the dLS. Drawing in a was inspired by ref. 49. c, More ventral, showing the projection from dCA2 to LS and to the ventral hippocampus. d, e, Diagram and enlarged view of a coronal dLS section from an Amigo2-Cre mouse brain injected into dCA2 with rAAV5-mGFP-p2A-synatophysin-mCherry, labelling dCA2 projections (green) and presynaptic terminals (red). f–h, Coronal sections of an Amigo2-Cre mouse brain injected unilaterally with rAAV5-EF1a-DIO-hChR2(E123T/T159C)-eYFP into the right CA2 area. f, Hippocampal section. g, h, LS sections. Three mice were injected per experiment. All mice presented similar staining patterns. Scale bars, 500 µm except e, 20 µm.
