Extended Data Fig. 2: Gating strategy for FACS analysis.
From: Structure of Plasmodium falciparum Rh5–CyRPA–Ripr invasion complex
a, Representative flow cytometry plots of unstained JK-1 cells and cells stained with anti-CD147-APC. Populations of cells were gated using forward and side scatter (top). Doublet exclusion was performed using FSC-A and FSC-H (middle). The voltage used for the APC channel (anti-CD147) was set using unstained cells, where the negative population was positioned < 103 (bottom). Experiments were repeated three times independently and were reproducible. b, Representative flow cytometry plots of JK-1 and JK-1ΔBSG cells in the presence or absence of recombinant proteins. Cells were incubated with no protein, single recombinant protein, the binary Rh5–CyRPA complex or the ternary Rh5–CyRPA–Ripr complex, followed by the subsequent incubation of the respective primary and secondary antibodies as indicated. Experiments were repeated three times with biologically independent samples, and were reproducible.
