Extended Data Fig. 4: Cryo-EM single-particle analysis of Rh5–CyRPA–Ripr complex
From: Structure of Plasmodium falciparum Rh5–CyRPA–Ripr invasion complex
a, A micrograph, after drift correction and dose-weighting. b, Reference-free 2D class averages. c, 3D classification resulted in separation of the binary CyRPA–Ripr complex (left) and the ternary Rh5–CyRPA–Ripr complex (right). d, FSC curves indicating the overall resolutions of the ternary Rh5–CyRPA–Ripr (blue) and binary CyRPA–Ripr (red) reconstructions. e, FSC curves between the final refined Rh5–CyRPA–Ripr ternary model and full map, excluding an unbuilt region of Ripr density (black); between the model refined in half map 1 and the reconstruction from that same half (FSCwork, blue); and between the model refined in half map 1 and the reconstruction from half map 2 (FSCtest, red) for the Rh5–CyRPA–Ripr ternary complex. f, g, Local-resolution estimation colour spectrum of the ternary Rh5–CyRPA–Ripr map (f) and the binary CyRPA–Ripr map (g).
