Extended Data Fig. 7: Transcriptional regulation of g-beige adipocyte differentiation.

a, mRNA expression of Gabpa, Erra, and Errg in GFP⁺ and GFP⁻ progenitors in the inguinal WAT of Myod1-Cre^ERT2 reporter mice treated with β-blocker. n = 4. b, mRNA expression of indicated genes in GFP⁺ and GFP⁻ beige fat from Myod1-Cre^ERT2 reporter mice. n = 3. c, Protein expression of PRDM16, EHMT1 and CKIIα in GFP⁺ and GFP⁻ progenitors in the inguinal WAT of Myod1-Cre^ERT2 reporter mice. β-actin was used as a loading control. Molecular mass (kDa) is shown on the right. d, mRNA expression of indicated genes in C2C12 myoblasts expressing empty vector, GABPα, ERRα or ERRγ using lentivirus. n = 3. e, mRNA expression of Myod1 in C2C12 myoblasts expressing an empty vector, GABPα, ERRα or ERRγ by lentivirus. n = 4. f, Protein expression of PPARγ and UCP1 in differentiated C2C12 cells expressing an empty vector or GABPα in pro-adipogenic conditions. β-actin was used as a loading control. Blots represent five independent samples. g, mRNA expression of Prdm16 in C2C12 myoblasts expressing empty vector, GABPα, ERRα or ERRγ using lentivirus. Differentiated immortalized beige adipocytes are included as a reference. n = 4. a, b, Data are mean ± s.e.m. of biologically independent replicates; unpaired two-sided Student’s t-test. d, e, g, Data are mean ± s.e.m. of biologically independent replicates; ANOVA followed by Tukey’s test.