Extended Data Fig. 1: Mouse models with defects in β-AR signalling.
From: Thermal stress induces glycolytic beige fat formation via a myogenic state
a, Schematic of the experiment. The inguinal WAT from wild-type and β-less mice at 23 °C or 15 °C was collected and analysed by RNA-seq. b, mRNA expression of indicated genes in interscapular BAT (iBAT) of mice in Fig. 1a. n.s., not significant. n = 5. c, GO analysis of gene set I in Fig. 1a. P values (−log10) by delta-method–based test. d, mRNA expression of gene set I: Ucp1, n = 10; Elovl3, n = 5; Cidea, n = 5; Pgc1a, n = 5; Cox8b, n = 5. e, mRNA expression of skeletal muscle-related genes in inguinal WAT. Myh1, n = 9; Myl1, n = 8; Mylpf, n = 8; Mybpc1, n = 9; Acta1, n = 8. f, mRNA expression of myogenesis-related genes in the iBAT of mice in a. n = 5. g, mRNA expression of indicated genes in the skeletal muscle of mice in a. n = 5. h, Schematic of the experiment. Wild-type mice were treated with β-blocker or vehicle (saline) at 23 °C for 5 days. i, mRNA expression of indicated genes in inguinal WAT (left), iBAT (middle) and skeletal muscle (right) of mice treated with β-blocker or vehicle. *P < 0.05. n = 4. Data are mean ± s.e.m. of biologically independent samples; unpaired two-sided Student’s t-test. j, Immunofluorescent staining of MHC in differentiated SVFs from the iBAT of mice in h. k, Immunofluorescent staining of MHC in differentiated SVF cells from the epididymal WAT of mice in h. l, Schematic of the experiment. SVFs from β-blocker-treated Myod1-CreERT2 reporter mice were cultured. b, d–g, Data are mean ± s.e.m. of biologically independent samples; one-way ANOVA followed by Tukey’s test. j, k, DAPI for counter staining. Images represent three independent replicates. Scale bar, 50 µm.
