Extended Data Fig. 4: MyoD+ progenitors in inguinal WAT.
From: Thermal stress induces glycolytic beige fat formation via a myogenic state
a, Schematic of the experiment. BrdU was administered in Myod1-CreERT2 reporter mice during β-blocker treatment. b, Immunofluorescent staining of BrdU and GFP in the inguinal WAT-derived SVFs from Myod1-CreERT2 reporter mice. Scale bar, 50 µm. Enlarged image, scale bar, 10 µm. c, Schematic of the experiment. GFP+ and GFP− progenitors were isolated from lineage-negative (Lin−) stromal cells in the inguinal WAT of Myod1-CreERT2 reporter mice by fluorescence-activated cell sorting. d, Transcriptome analysis in c. Each type of progenitor was pooled from 3 mice. Cut-off values was P < 0.05 by the delta-method-based hypothesis test. e, PCA of transcriptome dataset from indicated cells. Transcriptome of FAPs (GSE86073) is included in the analysis. f, Immunofluorescent staining of MyoD in isolated GFP+ progenitors in c. Note that all the GFP+ cells express MyoD protein. Scale bar, 100 µm. g, Immunofluorescent staining of MyoD and PAX7 in the SVFs from the inguinal WAT of Pax7-CreERT2;Rosa26-tdTomato reporter mice. Pax7 lineage cells were marked with tdTomato. Scale bar, 20 µm. h, Immunofluorescent staining of MyoD and PAX7 in the SVFs from iBAT of Pax7-CreERT2;Rosa26-tdTomato reporter mice in g. Scale bar, 10 µm. b, f–h, DAPI was used as counter stain. Images represent three independent experiments.
