Extended Data Fig. 2: RiboMethSeq analysis of 2′-O-methylated sites detected in HIV RNA isolated from viral particles.
From: FTSJ3 is an RNA 2′-O-methyltransferase recruited by HIV to avoid innate immune sensing
RiboMethSeq data are presented for each of the seventeen 2′-O-methylated sites. Data are superposed with relative local cleavage profiles for in vitro T7 transcripts of HIV-1 RNA, WT-HIV-1 RNA, siFTSJ3-HIV-1 RNA and CRISPR–Cas9-FTSJ3-HIV-1 RNA extracted from virions. Individual points for biological replicates are shown; n = 2 or n = 3. Position numbers are indicated on the bottom. In RiboMethSeq, the protection caused by 2′-O-methylation appears at the 3′-neighbouring position in RNA. For simplicity, a shift of –1 was applied to numbering here, so that the increased protection signal falls exactly to the 2′-O-methylated nucleotide.
