Extended Data Fig. 9: Metabolite alterations in complex III-deficient Treg cells.
From: Mitochondrial complex III is essential for suppressive function of regulatory T cells
a, NAD+/NADH ratio in Treg (CD4+Foxp3–GFP+tdTomato–RFP+) cells isolated from QPC wild-type (n = 5) or QPC iKO (n = 6) mice six weeks after receiving three doses of tamoxifen. b, Calculated intracellular concentration of 2-HG, succinate, α-KG and fumarate in Treg (CD4+Foxp3–GFP+tdTomato–RFP+) cells isolated from QPC wild-type (n = 5) or QPC iKO (n = 5) six weeks after receiving three doses of tamoxifen. c, d, Specific complex III-dependent (c) and TCA cycle (d) metabolites in CD4+Foxp3–YFP+CD25+ cells isolated from three-week-old RISP KO and RISP wild-type mice, quantified by LC–MS. Data are mean ± s.d.; two-tailed t-test (a, b;**P < 0.01; P values in Source Data); multiple two-tailed t-tests using a two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli, with Q = 0.01 (c, d). Each metabolite was analysed individually, without assuming a consistent s.d. (*Q < 0.01, **Q < 0.001, Q values in Source Data). All data points on graphs represent individual mice isolated and analysed on at least two separate days.
