Extended Data Fig. 2: Mice with regulatory T cells deficient in RISP do not display thymic dysfunction early in life.
From: Mitochondrial complex III is essential for suppressive function of regulatory T cells
a, b, Thymic weight (a) and total thymocyte number (b) observed in 10-day-old RISP KO (n = 5) and RISP wild-type (n = 5) mice. c, Absolute cell numbers of double-negative (DN; CD4−CD8−), double-positive (DP; CD4+CD8+), CD8 single-positive (CD8 SP; CD4−CD8+) and CD4 single-positive (CD4 SP; CD4+CD8−) populations from the thymuses of 10-day-old RISP KO (n = 5) and RISP wild-type (n = 5) mice. d, Foxp3–YFP+ and Foxp3–YFP+ CD4 SP absolute cell numbers in the thymuses of 10-day-old RISP KO (n = 5) and RISP wild-type (n = 5) mice. e, Representative contour plot of the Treg cell compartment in the superficial lymph nodes at three weeks of age. Contour plots are representative of at least three independent experiments with a total of at least five mice. Numbers in dot plot quadrants indicate percentage of cells. Data are mean ± s.d.; two-tailed t-test (a, b; P values in Source Data); multiple two-tailed t-tests using a two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli, with Q = 0.01 (c, d). Each cell type was analysed individually, without assuming a consistent s.d. (Q values in Source Data). All data points on graphs represent individual mice isolated and analysed on at least two separate days.
