Extended Data Fig. 5: Integrin β7 guides leukocytes to gut intraepithelium.

a, Top, representative histology staining for CD3 in small intestines of wild-type and β7^−/− mice. Bottom, quantification of CD3⁺ cells in each villus (more than 15 villi were counted for each mouse). ***P < 0.001, two-way ANOVA. b, Schematic of the competitive transfer experiments. Mice (CD45.2⁺) were lethally irradiated and transplanted with a 1:1 ratio mix of GFP⁺β7^−/− bone marrow cells and CD45.1⁺ wild-type bone marrow cells. The chimerism in different tissues was normalized to the ratio in blood. n = 5 mice. c, Representative flow cytometry plots and quantification of B cells and myeloid cells in mice depicted in b. n = 5 biologically independent recipients. d, Quantification of γδT cells in the liver. n = 3 wild-type mice; n = 4 β7^−/− mice. WBC, white blood cells. e, Quantification of γδT cells in the pancreas. n = 4 wild-type mice; n = 5 β7^−/− mice. f, Wild-type mice were lethally irradiated and reconstituted with bone marrow cell mixtures of β7^−/− and wild-type (β7^−/−:wild-type cells, 1:1 ratio) or β7^−/− and indicated knockout mice (β7^−/−:knockout cells, 1:1 ratio). The indicated mixed chimaeras that specifically lack intestinal B cells (β7^−/−μMT) or myeloid cells (β7^−/−Ccr2^−/−) were subjected to oral glucose-tolerance tests and the AUCs are shown. n = 5 mice per group for β7^−/− wild-type and β7^−/−μMT mice; n = 4 mice per group for β7^−/− wild-type and β7^−/−Ccr2^−/− mice. Data are mean ± s.e.m.

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