Extended Data Fig. 8: Cryo-ET of Rubisco–35 and Rubisco–M24-1/2 condensates.
From: Rubisco condensate formation by CcmM in β-carboxysome biogenesis
a, Analysis of the Rubisco–M35 condensate by cryo-ET. A slice through a tomographic volume of the matrix (left) and Rubisco–SSUL particles, from single-particle reconstruction with C1 symmetry and low-pass filtered to 40 Å, rendered into a 3D matrix (right). The circled cluster of complexes is shown again in Fig. 4e. A representative tomogram is shown (n = 3 independent tomograms of the same biological sample). b, Distribution of nearest neighbour centre-to-centre distances between Rubisco particles in tomograms of Rubisco–M35 (2,654 particles analysed) and Rubisco–M24-1/2 (1,405 particles analysed). Data are from n = 3 tomograms for each sample. The schematic above the histogram shows the distance between two Rubisco particles relative to the maximum distance spanned by the linkers between SSUL modules in M35. c, Analysis of the Rubisco–M24-1/2 condensate by cryo-ET. A slice through a tomographic volume of the matrix (left) and Rubisco–SSUL particles, from single-particle reconstruction with C1 symmetry and low-pass filtered to 40 Å, rendered into a 3D matrix (right). A representative tomogram is shown (n = 3 independent tomograms of the same biological sample).
