Extended Data Fig. 5: Sapienate is elongated to cis-8-octadecenoate.
From: Evidence for an alternative fatty acid desaturation pathway increasing cancer plasticity
a, Relative cis-8-octadecenoate abundance in cancer cells, normalized to T47D cells. HUH7, n = 3; A549, n = 3; H460, n = 5; DU145, n = 3; MDA-MB-468, n = 5; T47D, n = 5. One-way ANOVA with Tukey’s multiple comparisons. b, c, Relative cis-8-octadecenoate abundances in HUH7 and A549 control (non-targeting shRNA) and FADS2 knockdown (one of two FADS2 shRNAs) cells in control condition (ethanol) or upon supplementation with 20 μM sapienate, normalized to control. HUH7, control, n = 6 (ethanol) or n = 3 (sapienate); shFADS2-1, n = 3; shFADS2-2, n = 6 (ethanol) or n = 3 (sapienate); A549, control, n = 6; shFADS2-1, n = 3; shFADS2-2, n = 3. Data values are shown in Supplementary Table 1c, d. Two-way ANOVA with Tukey’s multiple comparisons. d, Relative proliferation of MDA-MB-468 cells with ethanol (n = 9) or 20 μM cis-8-octadecenoate (n = 3) upon treatment with DMSO or 0.5 nM Merck Frosst compound 3j. Data are normalized to control, with error bars representing s.e.m. Two-way ANOVA with Tukey’s multiple comparisons. e, f, Relative proliferation of HUH7 and A549 control (non-targeting shRNA) and knockdown (shFADS2) cells with ethanol or 20 μM cis-8-octadecenoate upon treatment with DMSO or 2 nM Merck Frosst compound 3j. HUH7, control, n = 9; shFADS2-1, n = 6; shFADS2-2, n = 9; A549: EtOH, n = 6; cis-8-C18:1, n = 3. Data are normalized to control. Two-way ANOVA with Tukey’s multiple comparisons. Only statistics for pairwise comparisons are depicted. Cancer cell experiments were performed in low FBS DMEM (1%, HUH7; 0.5%, all other cancer cells) with a treatment of 72 h. Error bars represent mean ± s.d. from biologically independent samples, unless otherwise noted.
