Extended Data Fig. 9: Inhibition of PUFA synthesis prevents ferroptotic cell death in ALCLs.
From: Squalene accumulation in cholesterol auxotrophic lymphomas prevents oxidative cell death
a, Immunoblotting of ACSL4 in the indicated Karpas299 cells. Actin is used as a loading control (top). Relative fold change in cell viability of indicated Karpas299 cell lines in the presence or absence of ML162 (20 nM) and Fer-1 (1 μM) for 5 days (bottom) (mean ± s.d., n = 3 biologically independent samples). Statistics, two-tailed unpaired t-test. b, Correlation of mRNA levels of ACSL4 with SQLE (left) and ALK (right) in primary ALCLs dataset (n = 22 biologically independent samples). c, Correlation of mRNA levels of ACSL4 with SQLE in CCLE dataset (n = 935 independent cell lines). d, Lipid peroxidation assessed by flow cytometry measuring C11-BODIPY fluorescence of indicated Karpas299 cell lines after an 18-h treatment with ML162 (200 nM). Representative data from one of three experiments are shown. e, Lipid peroxidation assessed by flow cytometry measuring C11-BODIPY fluorescence of indicated Karpas299 cell lines after an 18-h treatment in the presence and absence of ML162 (200 nM) and Fer-1 (1 μM).
