Extended Data Fig. 7: AKNA effects on cilia formation or localization, the upstream regulation of Akna, and summary of AKNA effects.
From: The centrosome protein AKNA regulates neurogenesis via microtubule organization
a, b, Histograms depicting the percentages of ciliated cells (ARL13+) (a, n = 4 independent experiments) and short versus long cilia (b, n = 4 independent experiments), in E14 primary cortical cells transfected with control shRNA or Akna shRNA. Short cilia are defined as dot-like (short axoneme or cilium not fully exposed to cell surface), and long cilia are defined as rod-shaped (long axoneme exposed at the cell surface). c, d, Integrated correlative light and electron microscopy micrographs of in utero-electroporated cells (n = 2 embryos). The images compare two neighbouring NSCs; one electroporated (blue) and one non-electroporated (orange). Yellow and pink arrows show anti-GFP and anti-TUBA immunogold signal in the cytoplasm and cilium of the electroporated cell. Notice that AKNA electroporation does not notably affect cilia formation in vivo. e, Confocal micrographs of CAMSAP3, CDH2 (N-cadherin) and F-actin staining in E14 cortex. i, Model describing the expression and functional role of AKNA in delamination and seeding of the SVZ. j. Schematic indicating and summarizing the modes of AKNA function in NSC delamination. Data in a, b are presented as mean ± s.e.m. Scale bars, 30 μm (c, immunofluorescence), 1 μm (c, electron microscopy), 0.1 μm (d), 25 μm (e).
