Extended Data Fig. 7: ASO knockdown of PSMD9 in C57BL/6J and DBA/2J mice.

a–c, Assessment of hepatotoxicity as measured by plasma levels (U/L) of aspartate transaminase (AST) and alanine transaminase (ALT) (a), percentage of liver weight to body weight (b) and total body weight (c) of mice on a normal chow diet and treated with PBS, control ASO or PSMD9 ASO for 7 days (n = 4 per group, twice-weekly injection at 25 mg kg⁻¹). d, Lipidomic analysis of total diacylglycerols and triacylglycerols in the plasma of mice on a chow diet (n = 4 C57BL/6J, n = 3 DBA/2J mice per group) or a Western diet (n = 6 mice per group, except n = 5 DBA/2J control ASO mice per group) and treated with either control or PSMD9 ASOs (twice-weekly ASO injection at 25 mg kg⁻¹). e–g, Assessment of hepatotoxicity as measured by plasma AST and ALT levels (e), percentage change in body weight from baseline (f), and food consumption normalized to body weight (g) from in vivo de novo lipogenesis experimental animals (n = 6 control ASO, n = 8 PSMD9 ASO, 28 days on diet and weekly injection of ASO injection at 25 mg kg⁻¹). *P < 0.05, **P < 0.01 control ASO versus PSMD9 ASO, t-test. Data are mean ± s.e.m.