Extended Data Fig. 3: Representative negative-stain electron micrographs.
From: Structural basis for KCTD-mediated rapid desensitization of GABAB signalling
a, The full SDS–PAGE gel for the pull-down experiment shown in Fig. 2a. b, Gαi competes with KCTD12 for binding of Gβ1γ2. These pull-down experiments were carried out as for Fig. 2a, except that Gαi was used instead of Gαq. c, Isothermal titration calorimetry affinity measurements for the binding of KCTD12H1 to Gβ1γ2. d, Representative negative-stain electron-microscopy two-dimensional image of KCTD16BTB+H1 bound to the C-terminal GABAB2 domain. e, Representative negative-stain electron-microscopy two-dimensional image of KCTD12H1/Gβ1γ2. f, Representative negative-stain electron-microscopy two-dimensional image of KCTD12BTB+H1/GABAB2Cter/Gβ1γ2.
