Extended Data Fig. 7: GGT5 is expressed by mouse FDCs.
From: S-Geranylgeranyl-l-glutathione is a ligand for human B cell-confinement receptor P2RY8
a, Violin plots from a single-cell RNA sequencing dataset18, showing the relative expression levels of Ggt1, Ggt5, Ggt6 and Ggt7 in the indicated stromal cell (SC) subsets. MRC, marginal reticular cell; PvC, perivascular cell; TRC, T zone reticular cell. b, qPCR for expression of Ggt1, Ggt5, Ggt6 and Ggt7 in whole spleen tissue or spleen stroma, relative to Hprt (n = 3 biological replicates). c, d, RNAscope detection of Ggt5 mRNA (red) counterstained with IgD (brown) in the indicated tissues (spleen, peripheral lymph nodes (LN) and Peyer’s patch) in mice eight days after immunization with SRBCs (c) or in lymph nodes from mice treated with LTβR–Fc and TNFR–Fc fusion proteins or control IgG for four days (d). Serial sections are stained for CR1 (blue) and IgD (brown). Scale bars, 100 µm. Each point in b corresponds to a biological replicate. Data are representative of five (c), two (d) or one (a) biological replicates per condition. Graphs depict mean with s.d. The violin plots in a were generated by a webtool (http://scorpio.ucsf.edu/shiny/LNSC/) that does not display the exact minimum, maximum, centre, percentiles or n numbers for each group.
