Extended Data Fig. 8: Controls for transduced B cell co-transfer experiments.
From: S-Geranylgeranyl-l-glutathione is a ligand for human B cell-confinement receptor P2RY8
a, Immunofluorescence images tracking the positioning of adoptively transferred B cells overexpressing empty vector–GFP (green), and co-transferred with either empty vector–Thy1.1- or GGT5–Thy1.1-overexpressing B cells, in unimmunized (top) or SRBC-immunized (bottom) mice, relative to endogenous B cells (IgD, blue). b, Quantification of images of the type in a and in Fig. 4h, measuring the average distance (in arbitrary units, A.U.) of GFP+ cells from the centre of B cell follicles using IMARIS software. Each point represents a B cell follicle, and three to four similarly sized follicles were chosen randomly from three mice per condition (n = 10 follicles per condition). Graph depicts mean with s.d. P values determined by one-way ANOVA with Bonferroni’s multiple comparisons test. c, Immunofluorescence images tracking positioning of adoptively transferred B cells overexpressing GGT5 or an empty vector control construct from immunized mice of the type in Fig. 4h, by staining for Thy1.1 (red) relative to endogenous B cells (IgD, blue). Scale bars, 100 µm. Data are representative of three (a, c) biological replicates per condition.
