Extended Data Fig. 4: GGG specifically inhibits migration of P2RY8-expressing WEHI-231 cells.
From: S-Geranylgeranyl-l-glutathione is a ligand for human B cell-confinement receptor P2RY8
a, Representative flow cytometry plots of migration-inhibition assays performed with 50 ng ml−1 CXCL12 and 100 nM GGG on WEHI-231 cells transduced with empty vector-GFP, P2RY8-GFP, S1PR2-Thy1.1 or GPR4-Thy1.1. b, Transwell migration-inhibition assay using 500 ng ml−1 CXCL13 and the indicated amounts of GGG for WEHI-231 cells transduced with P2RY8-GFP and empty vector-GFP (n = 4). c, Summarized data for WEHI-231 cells transduced with S1PR2-Thy1.1, GPR4-Thy1.1 and empty vector-GFP from assays of the type in a (S1PR2 and GPR4, n = 3; empty vector, n = 4). Data are representative of two independent experiments (a) and pooled from two independent experiments (b, c). Graphs depict mean with s.d. and points represent biological replicates.
