Extended Data Fig. 9: IL-6–STAT3–SAA signalling axis does not affect expression of MIF and TIMP1.
From: Hepatocytes direct the formation of a pro-metastatic niche in the liver
a, Study design for b–e (n = 5 and 4 for mice injected with PBS and PDAC cells). b, c, Images of CK19 (yellow) and TIMP1 (purple) in the pancreas, primary tumour, and liver. Data representative of at least three independent experiments (a–c). d, FPKM values of genes in the liver of control mice (n = 5) and NTB KPC mice (n = 5) obtained from QuantSeq 3′ mRNA sequencing. Data represented as box plots (centre line, median; box limits, upper and lower quartiles; whiskers, max and min values). e, mRNA levels of Timp1 in the liver of control mice (n = 6), NTB KPC mice (n = 7), and tumour-bearing KPC mice (n = 6) relative to Actb. Data representative of one experiment (d, e). f, j, n, Study designs for g–i, k–m and o–q, respectively. For f, n = 5 for Il6+/+ mice injected with PBS and n = 5 or 6 for Il6+/+ mice injected with PDAC cells. n = 4 and 5 for Il6−/− mice injected with PBS and PDAC cells, respectively. For j, n = 5 for all groups, except n = 4 or 5 for Saa+/+ injected with PDAC cells. For n, n = 4 for Stat3flox/flox mice and n = 8 and 7 for Stat3flox/flox Alb-cre mice injected with PBS and PDAC cells, respectively. g, k, o, mRNA levels of Mif and Timp1 in the indicated organs relative to Actb. h, l, p, Images of CK19 (yellow) and TIMP1 (purple) in the pancreas and primary tumour. i, m, q, Concentration of TIMP1 in the serum. Data representative of one experiment (f–q). Scale bars, 50 μm. Statistical significance calculated using one-way ANOVA with Dunnett’s test (i, m, q) or two-tailed Mann–Whitney test (other panels). NS, not significant. ND, not detected. Data represented as mean ± s.d. unless indicated otherwise.
