Extended Data Fig. 5: Heterogeneity of biomarkers that predict responses to checkpoint blockade.

a, The TIDE gene-signature score of each tumour region is shown per patient, for patients with more than one region available (n = 39). Using a threshold shown by the dashed line, patients are classified as having high TIDE (light blue), low TIDE (dark blue) or heterogeneous TIDE (orange) scores. b, The IPRES gene-signature score of each tumour region is shown per patient, for patients with more than one region available (n = 39). Using the previously defined threshold¹³ (shown by the dashed line), patients are classified as having low IPRES (light blue), high IPRES (dark blue) or heterogeneous IPRES (orange) scores. c, The expanded Ayers et al.¹⁴ IFN signature is shown for each tumour region per patient, for patients with more than one region available (n = 38). For a–c, the immune classification of the patient is also given. d, The greatest difference in the expanded Ayers et al.¹⁴ IFN signature between tumour regions from the same tumour is plotted, according to whether or not the tumour has heterogeneous levels of immune infiltration (n = 38). A two-sided Wilcoxon rank-sum test is used for comparison. e, Tumour mutational burden of each tumour region is shown per patient (n = 93). Using a threshold of ten mutations per megabase (dashed line), patients are classified as having a low (light blue), high (dark blue) or heterogeneous tumour mutational burden (orange). For all box plots, the minimum and maximum are indicated by extreme points of the plot; the median is indicated by a thick horizontal line; and the first and third quartiles are indicated by box edges. f, A summary of the tumour histology, immune classification, tumour mutational burden status, TIDE category and IPRES category is shown for each tumour (n = 93). There is an enrichment for heterogeneously immune infiltrated tumours to have heterogeneous tumour mutational burden status and heterogeneous TIDE scores (Fisher’s exact test). No corrections were made for multiple comparisons.