Extended Data Fig. 8: Automated identification of TAD boundaries from insulation score.
From: Visualizing DNA folding and RNA in embryos at single-cell resolution
Relative distance maps for each segment, plotted at 3-kb resolution. TAD boundaries (dotted black lines) were identified by an automated algorithm based on computation of the insulation score (plotted below each graph) and application of a threshold. A threshold score of 0.4 was used to call TAD boundaries, which agree with the boundaries detected by manual inspection and annotated as in Fig. 4. The insulation score for each segment was computed as follows: for each barcode, we first measured a score for the upstream region by computing the fraction of red bins (normalized distance <0) in a block of 6 × 6 bins and a similar score for the downstream block and for the interblock region. To compute the insulation score, we then took the average scores for the two blocks minus the score of the interblock.
