Extended Data Fig. 9: Comparison of 3-kb segment-specific datasets.

a, Normalized distance maps from dataset 1, embryos 10–12 hpf. b, Normalized distance maps from dataset 2, embryos 8–10 hpf, showing similar patterns to dataset 1. All maps are normalized relative to cells from the head segments, as in Fig. 4. c, Pearson correlation between datasets 1 and 2 by segment. Note that after normalization, organization in the head and anterior thorax is apparently random, and thus uncorrelated between datasets. The systematic features that appear in posterior segments, in which regulatory sequences of the BX-C are active, are similar between datasets. d, Pearson correlation of non-normalized distance data. In c and d, values from all unique combinations of the 52 barcodes were used to compute the correlation.