Extended Data Fig. 5: In vivo activity of pre-LC^PDYN neurons after ingestive behaviours.

a, Placement of an implanted optic fibre and GCaMP6s expression in the pre-LC. Scale bar, 50 μm. b, A low concentration (0.06 M) of NaCl had inhibitory effects on pre-LC^PDYN neurons (n = 7). c, Licking an empty spout had no inhibitory effect on pre-LC^PDYN neurons (n = 4 for eYFP, n = 4 for GCaMP6s). d, Peristimulus time histogram of GCaMP signals around the start of sodium ingestion. Data were magnified from c and Fig. 3a. Fluorescence changes (ΔF/F) from −1 to 0 s were calculated. e, Activity change per lick was quantified for Fig. 3d and e. *P < 0.05, **P < 0.01 by two-tailed Wilcoxon or two-tailed Mann–Whitney U-test. Data are mean ± s.e.m.