Extended Data Fig. 2: Sodium appetite induced by the photostimulation of pre-LC^PDYN neurons.

a, Photostimulation of pre-LC^PDYN neurons increased the intake of a lower concentration of NaCl (0.06 M and 0.15 M, n = 5 for eYFP, n = 4 for ChR2). b, Photostimulation triggered sodium appetite in both sexes (left, n = 7 female, n = 4 male), at any time of the day (right, n = 7). Data were partially reanalysed from Fig. 1e and g. c, Left, pre-LC^PDYN-stimulated animals favoured NaCl over KCl (n = 9). Right, NaCl consumption was reduced in the presence of amiloride (n = 8). 0.5 M solutions were used for NaCl and KCl. d, Left, representative plots showing lick events during the 5 s of water or KCl access. Right, the effect of amiloride on water and KCl intake was quantified under water deprivation and sodium depletion. The total number of licks from five trials with amiloride was averaged and divided by that without amiloride (n = 9). *P < 0.05, **P < 0.01, ***P < 0.001 by two-tailed Mann–Whitney U-test or Friedman test (with Dunn’s multiple comparison). Data are mean ± s.e.m.