Extended Data Fig. 10: Depletion of the members of COMPASS complex impairs GCR in capn3a^−/− embryos.

a, Statistical analysis of the average liver sizes in wild-type, capn3a^−/−, wild-type embryos injected with ST-MO, capn3a-MO or wdr5-MO, and capn3a^−/− embryos injected with ST-MO or wdr5-MO at 3.5 dpf. b, qRT–PCR analysis of capn8 and capn12 mRNA expression in wild-type and capn3a^−/− embryos injected with ST-MO or wdr5-MO as shown. The injection of ST-MO was used as a negative control and given a value of one. c, WISH of capn8 in the wild-type and capn3a^−/− embryos at around 1 dpf. Left, wild-type and capn3a^−/− embryos without injection. Right, wild-type and capn3a^−/− embryos with wdr5-MO injection. Red arrow denotes the region with upregulated capn8 expression; green arrow denotes the region with downregulated capn8 expression after wdr5-MO injection. d, Relative mRNA expression of capn8 in wild-type embryos injected with or without wdr5-MO, the wdr5-overexpression plasmid (wdr5-p), or wdr5-MO together with wdr5 plasmid, and in capn3a^−/− embryos injected with or without wdr5-MO, the wdr5-overexpression plasmid (wdr5-p), or wdr5-MO together with wdr5 plasmid. e, Schematic diagram showing the genomic structure and a genetic mutation of the zebrafish wdr5 gene. ATG denotes the translation start codon; black lines denote introns; vertical bars denote exons (blue for UTR regions, black for the coding region). The red underlined sequence denotes the additional DNA (10 bp) in the wdr5 mutant; the numbers in the fifth and sixth panels denote the amino acid positions of one domain in zebrafish Wdr5 protein, and the length of predicted Wdr5 mutant protein (Wdr5ⁱ¹⁰). f, WISH was performed to assess the liver sizes in 3.5-dpf-old wild-type and capn3a^−/− embryos injected with different reagents as indicated. Ctr, uninjected control. g, Statistical analysis of the average liver sizes in f under the different treatments as indicated. h, Relative mRNA expression of capn8 and capn12 in 1.5-dpf-old wild-type and capn3a^−/− embryos with different treatments as indicated. The injection of ST-MO was used as a negative control and given a value of one. i–k, qRT–PCR analysis of capn8 and capn12 mRNA expression in wild-type and capn3a^−/− embryos injected with ST-MO or setd1a-2#MO (i), rbbp5-2#MO (j), or ash2l-2#MO (k). The injection of ST-MO was used as a negative control and given a value of one. Each experiment was repeated three times with similar results, and a representative result is shown. n indicates the number of zebrafish embryos in each group. Data are mean ± s.d. from three biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed t-test.