Extended Data Fig. 3: The GCR is differentially activated in nid1a genetic mutants.

a, Alignment of cDNA sequences between nid1a and its family members. The identity of cDNA sequences and the GeneBank accession number of each gene were provided. b, Relative mRNA expression of nid1a in wild-type and nid1a^d2/+ embryos at different times as indicated. c, Schematic diagram showing a nid1a mutant allele (nid1a-atg^del) with a deletion in the start codon (the first ATG). Black lines denote introns; black bars denote exons; ATG (surrounded by a black box) denotes the start codon; red horizontal line through the letters denotes deletion of DNA sequence in nid1a-atg^del mutant allele. d, Images of the wild-type and nida-atg^del larvae at 3, 4 and 5 dpf. e, Statistical analysis of the average body lengths in samples as shown in d. f, Relative mRNA expression of nid1a, nid1b, nid2a and nid2b in wild-type and nid1a-atg^del embryos at 1.5 dpf. Each experiment was repeated three times with similar results, and a representative result is shown. n indicates the number of zebrafish embryos in each group. Data are mean ± s.d. from three biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed t-test.