Extended Data Fig. 4: GCR is triggered by a transgene with a PTC and the nucleotide sequence homologous shared by the compensatory endogenous gene.

a, Relative mRNA levels of capn3a, capn8 and capn12 in wild-type and capn3a^−/− embryos injected with uncapped egfp RNA, capped capn3a RNA, uncapped capn3a RNA, capped capn3a^Δ14 RNA or uncapped capn3a^Δ14 RNA at 1.5 dpf. b, Relative transcript levels of the transgene in different transgenic lines injected with or without upf1-MO at 1.5 dpf as indicated. c, Relative transcript levels of the transgene in different transgenic lines treated with or without cycloheximide (CHX) at 3 dpf. d, Relative expression of endogenous nid1a mRNA in different transgenic lines at 3 dpf as indicated. Each experiment was repeated three times with similar results, and a representative result is shown. n indicates the number of zebrafish embryos in each group. Data are mean ± s.d. from three biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed t-test.