Extended Data Fig. 5: Knockdown of upf3a impairs GCR in both capn3a^−/− and nid1a^−/− embryos.

a, Relative mRNA expression of capn3a, capn8 and capn12 in wild-type and capn3a^−/− embryos treated with or without cycloheximide at 2 dpf. Mu, capn3a^−/− embryos. b, Top, WISH was performed to assess the liver sizes in wild-type and capn3a^−/− embryos injected with different reagents as indicated at 3.5 dpf. Bottom, statistical analysis of the average liver sizes. c, Relative mRNA expression of capn3a, capn8 and capn12 in wild-type and capn3a^−/− embryos injected with or without upf3a-MO at 1.5 dpf. d, Images of the wild-type and nida^Δ2/Δ2 mutant (designated nid1a^−/−) larvae injected with different reagents as indicated at 3, 4 and 5 dpf. e, Statistical analysis of the average body lengths in samples as shown in d. Each experiment was repeated three times with similar results, and a representative result is shown. n indicates the number of zebrafish embryos in each group. Data are mean ± s.d. from three biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed t-test.