Extended Data Fig. 2: Heart morphology and vascular characterization of mice after five weeks of different dietary regimens.
From: Nitrosative stress drives heart failure with preserved ejection fraction
a, Representative images of haematoxylin and eosin (H&E), WGA, Masson’s trichrome (MT) and lectin staining in transversal sections of left ventricle of mice of different experimental groups. Images are representative of four independently performed experiments with similar results. Scale bars, 500 μm (haematoxylin and eosin) and 50 μm (WGA, Masson’s trichrome and lectin). b, WGA quantification of cardiomyocyte cross-sectional area (n = 4 mice per group). c, Percentage of fibrosis area in Masson’s trichrome-stained transversal sections (n = 4 mice per group). d, Myocardial capillary density (n = 4 mice per group). e, Aortic PWV of mice of different experimental groups (n = 5 mice per group). f, Representative pulsed-wave Doppler tracings of coronary flow in mice fed with chow (top) or HFD + l-NAME (bottom) under basal conditions (left; 1.5% isofluorane (Iso)) and after hyperaemic stimulus (right; 3% isofluorane). Images are representative of five independent mice. g, Coronary flow reserve (CFR) quantification (n = 5 mice per group). Data are mean ± s.e.m. b–d, e, g, One-way ANOVA followed by Sidak’s multiple-comparisons test. Numbers above square brackets show significant P values.
