Extended Data Fig. 5: NRL estimation in BRG1 conditional-knockout cells.
From: Mammalian ISWI and SWI/SNF selectively mediate binding of distinct transcription factors
a, Scheme illustrating transposase insertion in chromatin. b, Mono-, di- and tri-nucleosomal fragment lengths estimated from smoothed and de-trended ATAC-seq fragment sizes (here showing wild-type replicate 1 as an example). The three estimates are averaged to obtain a single value per biological replicate. c, Estimated changes in nucleosome-repeat lengths based on ATAC-seq data from previous studies6,7 (as indicated) in BRG1 KO compared to controls. None of the datasets shows a significant increase in values (all P > 0.05, one-sided Welch two-sample t-test). d, e, MNase-based estimation of nucleosome repeat length upon BRG1 deletion using data from a previous study6. e, Counts of distances between same-strand MNase-seq alignments (phase) in Brg1fl/fl and BRG1 KO cells. e, Linear fits to the phase peaks from d and resulting nucleosome-repeat lengths with 95% confidence intervals. f, Genotyping PCR for floxed (2,507-bp product) and deleted (313-bp) BRG1 cells.
