Extended Data Fig. 5: LGR5 dynamics during organoid development.
From: Self-organization and symmetry breaking in intestinal organoid development
a, Representative images of organoids grown from LGR5+ and LGR5− starting populations at 0, 24 and 120 h of development. b, Cell types and proliferation markers plotted on pseudotime for LGR5+ (top, n = 9,798) and LGR5− (bottom, n = 13,623) starting populations. c, Light-sheet time-lapse imaging of organoid formation starting from a single LGR5+ cell (GFP signal) expressing membrane-bound mCherry (MEM9–mCherry). Green arrows are pointing to cells re-acquiring GFP signal from LGR5 reporter in the organoid crypts. d, Quantification of GFP signal of LGR5 reporter from time lapse shown in c. Cells localized in the organoid crypt are plotted in green, cells localized outside the crypt and on the main body are plotted in grey (n = 91 cells).
