Extended Data Fig. 7: YAP1 is necessary but not sufficient for organoid formation.
From: Self-organization and symmetry breaking in intestinal organoid development
a, Fraction of cells per organoid having YAP1 in the ON state (green, nuclear localization) or in the OFF state (red, cytoplasm localization) over time (n = 1,074 organoids). b, Entropy of YAP1 states (that is, variability in YAP1 activation in ON and OFF states) over time (n = 1,074 organoids). Shading denotes s.d. c, Quantification of YAP1 mean intensity after Wnt removal at 48 h or 72 h in organoids fixed at 72 h or 96 h (n = 4 replicates). Violin plot lines show quartiles. d, Images of organoids derived from single cells isolated from organoids at 72 h or at 120 h. e, Organoids treated at different time points with control or with verteporfin. Fixation at 96 h. Left, representative images; right, efficiency quantification (normalization: control, n = 2 replicates). Bar plots depict the mean. f, YAP1 overexpressing organoids with or without Wnt. Fixation at 96 h. Left, representative images; right, efficiency quantification (normalization: control, n = 4 replicates). P = 0.001, two-sided t-test. g, Left, images of organoids with Wnt removal at 48 h, or after double knockout of LATS1 and LATS2 (LATS DKO). Top, fixation at 72 h. Bottom, fixation at 96 h (Wnt removal at 48 h) or 120 h (LATS DKO). Right, quantification of enterocysts as retrieved in the left panel (n = 4 replicates for Wnt removal at 48 h; n = 3 replicates for LATS DKO). P = 0.0001 (Wnt removal); P = 0.002 (LATS DKO), two-sided t-test. h, Representative images of control, verteporfin treatment at 48 h, Wnt removal at 48 h, YAP1 overexpression, LATS DKO or treatment with EREG at 0 h. Top, fixation at 72 h; bottom, fixation at 96 h (Wnt removal at 48 h, YAP1 overexpression, LATS DKO) or 120 h (control, verteporfin 48 h, EREG). i, Scatter plot of Flag mean intensity signal and YAP1 mean intensity signal (left) and of Flag mean intensity and aldolaseB mean intensity (right) in YAP1-overexpressing organoids at 96 h. Data are mean ± s.d.
