Extended Data Fig. 8: Growth of lung adenocarcinomas after RB reactivation.
From: RB constrains lineage fidelity and multiple stages of tumour progression and metastasis
a, Imaging of individual tumour growth by μCT. Images were taken weekly starting 11 weeks after tumour initiation. RB reactivation (tamoxifen treatment) initiated at week 12. Average fold change after week 12 is shown. KP;RbTR/TR, n = 6 mice; KP;RbR/R, n = 6 mice. Data are mean ± s.d. Significance at 14 weeks was determined by unpaired two-tailed Student’s t-test. P = 0.2617. b, Representative μCT images quantified in a. c, Low-magnifiation scans of sections through tumour lobes showing relative tumour burden 2 weeks after RB reactivation in KP;RbTR/TR and KP;RbR/R cohorts. d, Quantitative PCR detection of the GFP cDNA within the RbTR allele. DNA templates for PCR were isolated by laser capture microdissection of individual tumours. KP;RbTR/TR, n = 8 tumours from 2 mice; KP;RbR/R, n = 8 tumours from 2 mice. Significance was determined by two-tailed unpaired Student’s t-test. P = 0.0023. Data are mean ± s.d.
