Extended Data Fig. 9: Impact of RB reactivation on proliferation, MAPK signalling and RB phosphorylation.
From: RB constrains lineage fidelity and multiple stages of tumour progression and metastasis
a, Ki67 immunohistochemistry of KP;RbTR/TR and KP;RbR/R tumours 3, 7 and 14 days after Rb1 restoration. b, Quantification of a. n = 10 tumours from 3 mice for 0-, 3- and 7-day time points; n = 15 tumours from 3 mice for the 14-day time point. Significance was determined by χ2 test for trend. P = 0.3289. c, Quantification of the percentage of total tumour cells that are Ki67+ in KP;RbTR/TR and KP;RbR/R tumours 14 days after Rb1 restoration. KP;RbTR/TR, n = 14 tumours from 1 mouse; KP;RbR/R, n = 14 tumours from 1 mouse. Significance was determined by two-tailed unpaired Student’s t-test with Welch’s correction. P = 0.0110. Data are mean ± s.d. d, Immunohistochemistry for RB, p-RB S807/S811 and p-ERK in KP;RbTR/TR and KP;RbR/R tumours 3, 7 and 14 days after Rb1 restoration. Scale bars, 100 μm; insets are magnified 5×. e, Contingency test for NKX2-1+HMGA2+ KP;RbTR/TR and KP;RbR/R tumours 2 weeks after Rb1 restoration. NKX2-1+HMGA2+ KP;RbTR/TR, n = 23 tumours from 2 mice; KP;RbR/R, n = 12 tumours from 2 mice. Significance was determined by two-sided χ2 test. P = 0.0019.
