Extended Data Fig. 6: Multinucleation and cardiomyocyte hypertrophy in miR-199a-treated pig hearts.

a, Representative images of longitudinal sections stained with WGA to assess the number of nuclei per cardiomyocyte in the infarct border zone of AAV6-control- and AAV6-miR-199a-treated animals (n = 4 and n = 6, respectively; analysis is from at least seven high-resolution images acquired from at least eight different regions of each heart), 12 days after MI. The right panels show the estimated number of nuclei for each cardiomyocyte. Scale bar, 50 µm. b, Additional representative images of mono- or bi-nucleated BrdU⁺ cardiomyocytes in the infarct border zone of AAV6-control- and AAV6-miR-199a-treated animals, 12 days after MI. Scale bar, 50 µm. c, Cross-sectional area measurements of BrdU⁺ and BrdU⁻ cardiomyocytes in AAV6-control- and AAV6-miR-199a-treated pigs 12 days after surgery. Data are mean ± s.e.m. from the analysis of four pigs. d, Representative images of BrdU⁺ and BrdU⁻ cardiomyocytes. Scale bar, 50 µm. The right panels are high-magnification images of the indicated portions of the left images.