Extended Data Fig. 1: Transduction of swine hearts after MI with AAV vectors.
From: MicroRNA therapy stimulates uncontrolled cardiac repair after myocardial infarction in pigs
a, b, AAV6 is the most effective serotype for porcine heart transduction. The graphs show viral genomes (a) and eGFP mRNA (b) levels one month after direct intramyocardial injection of 1 × 1012 viral genome particles of AAV6, AAV8 and AAV9 vectors carrying the eGFP transgene (these three AAV serotypes have been reported to transduce post-mitotic tissues at high efficiency28). Data are mean ± s.e.m.; the number of animals per group is indicated. c, Nucleotide sequence of the miR-199a-1 precursor. Mature miR-199a-5p and miR-199a-3p sequences are shown in green and their seed sequences are shown in blue and red, respectively. d, Mature miR-199a-5p and miR-199a-3p sequences are conserved in human, mouse, rat and pig. The miRNA seed sequences are shown in blue for miR-199a-5p and in red for miR-199a-3p. e, Representative photograph taken during porcine surgery and vector injection. After thoracotomy, the pericardial sac was opened, the LAD was exposed and occluded below its first branch for 90 min. Ten minutes after reperfusion, AAV6-control or AAV6-miR-199a were injected into the infarct border zone.
