Extended Data Fig. 9: The contribution of KRT20-expressing cells during development and homeostasis.
a, Detection of keratin 20 (KRT20, magenta), lysozyme1 (LYZ1, red) and GFP (Lgr5-DTR-eGFP) in adult mouse intestine. Tissue is counterstained with DAPI (cyan). Representative image of n = 3 biologically independent samples is shown. b, Detection of E-cadherin (cyan), GFP (green) and RFP (red) in tissue whole mounts from the proximal part of the small intestine isolated from Rosa26-lsl-Confetti;Krt20-T2A-creERT2 mice at 3 and 40 days after label induction with 4-hydroxytamoxifen. Samples from three mice were analysed per time point and representative images are shown. c, In situ hybridization of Lgr5 (green) and cre (red) in proximal E16.5 small intestine. Tissue is counterstained with DAPI (cyan). Samples from three mice were analysed per time point and representative images are shown. d, Detection of E-cadherin (cyan) and tdTomato (red) in tissue whole mounts from the proximal small intestine isolated from Rosa26-lsl-tdTomato;Krt20-T2A-creERT2 mice at E18.5 and adulthood following administration of 4-hydroxytamoxifen at E16.5. Representative images of n = 2 samples are shown.
