Extended Data Fig. 1: LGR5+ cells and proliferative cells are restricted to the intervillus regions.
a, Detection of E-cadherin (red) and GFP (Lgr5-DTR-eGFP) at E13.5 and E16.5 in proximal and distal small intestine. Tissue is counterstained with DAPI (cyan). A representative image from each time point and intestinal segment is shown (n = 3 mice analysed). b, In situ hybridization of endogenous Lgr5 at E13.5 and E16.5 in proximal and distal small intestine. Tissue is counterstained with DAPI (cyan). A representative image from each time point and intestinal segment is shown (n = 3 mice analysed). c, Detection of E-cadherin (magenta) and GFP (Lgr5-DTR-eGFP) in intestinal whole mount at E16.5 in proximal small intestine. A representative image from n = 3 independent samples is shown. d, Detection of Ki67 and EdU (red) following a 1-h pulse at the indicated time points (left and right, respectively). Three mice were analysed at each time point and a representative image is shown. Scale bars, 50 μm. e, f, Quantification of number and location of EdU+ cells as depicted (e) from at least ten intervillus regions per mouse at E16.5, P5, P11 and adult, and 20 intervillus regions per mouse at P0 up to position 10 and position 17 (adult). Samples from three mice were analysed at each time point. Data are mean ± s.e.m.
