Extended Data Fig. 8: The downregulation of Mitofusin in cysts is not mediated by known regulators of Mitofusin protein.
From: Mitochondrial fragmentation drives selective removal of deleterious mtDNA in the germline
a–h″, Germaria of females expressing haemagglutinin-tagged Mitofusin, under control of the Mitofusin promoter (Marf-gHA48) reacted with anti-haemagglutinin antisera to detect Mitofusin (greyscale in a–h, magenta in a″–h″) and anti-Vasa antibody to delineate the germline (greyscale in a′–h′, blue in a″–h″). The indicated known regulators of Mitofusin protein levels were knocked down in the germline using RNAi under the control of nos-GAL4. The numbers in parentheses are Bloomington Drosophila Stock Center stock numbers. All ovarioles are oriented with the stem-cell niche towards the left.
