Extended Data Fig. 2: SPR binding studies of human STING with TBK1.
From: A conserved PLPLRT/SD motif of STING mediates the recruitment and activation of TBK1
a, Domain organization of human STING and truncated forms of STING used in this study. b, SDS–PAGE analyses of human STING (left) and human or mouse TBK1 (right) used in the SPR studies. c–l, Top, SPR binding studies of human STING with human or mouse TBK1. Experiments without cGAMP in the running buffer are indicated. All others were conducted with 1 μM cGAMP. Bottom, the binding affinity (Kd) was determined by fitting the binding data to a one-site binding model. Data in b–l are representative of at least two independent experiments.
