Extended Data Fig. 1: Posterior intestinal cells have impaired perinuclear anchoring capabilities.
From: Active chromatin marks drive spatial sequestration of heterochromatin in C. elegans nuclei
a, Quantification of gwIs4 and gwIs42 reporters distribution in zone 1, as described in Fig. 1b, in compartments of the intestine of wild-type L1 larvae, as indicated. Strains used: GW1056 and GW447. For both reporters, posterior nuclei were compared pairwise to the rest of the intestine by χ2-test. ***P < 0.001. P and n (foci scored per condition) values are listed in Supplementary Table 1. b, Schematic of gwIs42, and cartoon showing that it gains peripheral localization during differentiation6. Focal section of 1 representative wild-type L1 larvae of 15 bearing gwIs4 and EMR-1–mCherry (left), and of 1 of 11 bearing gwIs42 and LMN-1–GFP (right). Insets show intestinal single nuclei as indicated. c, Single focal planes of 1 of 20 representative L1 larvae bearing gwIs4 and EMR-1–mCherry in wild type and mrg-1; cec-4 double mutants. Insets show enlarged single intestinal nucleus of the indicated compartment.
