Extended Data Fig. 3: H3K36me2/me3 depletion mimics MRG-1 loss.
From: Active chromatin marks drive spatial sequestration of heterochromatin in C. elegans nuclei
a, Quantification of H3K36 methylation levels in met-1 (GW1183) and mes-4 (RNAi) single- and double-depleted L1 larvae by quantitative mass spectrometry. Data are shown relative to wild-type L1 (N2) on control RNAi bacteria (dotted line, 100%) and as a mean of three biological replicates (shown as dots). b, L1 larvae of the indicated genotype stained for H3K36me2 and DAPI in strains GW554, GW566 and GW637. The image is representative of nine independent larvae. c, gwIs4 reporter distribution in zone 1 in indicated compartments of the intestine of strains GW1056, GW1041, GW1088, GW1133, GW1151 and GW1090 (genotypes indicated). d, Focal section of 1 representative L1 larvae of 18 bearing gwIs4 and EMR-1–mCherry in met-1; cec-4; mes-4 mutants. Insets show single intestinal nucleus of the indicated intestine compartment. e, Reporter distribution in zone 1 in hypoderm and intestine of the indicated genotypes (GW1056 and GW1090). Whole-intestine values reflect results pooled in relation to relative cell-type abundance in the tissue. In c and e, each sample was compared pairwise to wild type by χ2 test. *P < 0.05, **P < 0.01 and ***P < 0.001; P and n (number of foci scored) values are listed in Supplementary Table 1.
