Extended Data Fig. 5: MRG-1 and H3K36me genomic sites correlate with H3K27ac.
From: Active chromatin marks drive spatial sequestration of heterochromatin in C. elegans nuclei
a, Zone 1 gwIs4 reporter distribution in anterior intestine of mrg-1; cec-4 mutant L1 larvae (strain GW1038) with RNAi of control or of the indicated gene. Combined results of two biological replicates are shown. b, Schematic of CBP-1 and CBP-3. c, Mean zone 1 reporter distribution in (strain GW1037) control or RNAi treated cec-4 mutants. Dots represent three biological replicates. Each sample was compared pairwise to control RNAi by χ2 test. P and n (foci scored per condition) values are listed in Supplementary Table 1. d, mRNA expression of endogenous and overexpressed cbp-1 in L1s of the indicated genotypes analysed by RT–qPCR (strains GW1343, GW1345, GW1641, GW1658 and GW1646). The mean of two biological replicates is shown together with dots showing data distribution. e, mRNA expression analysis of the cbp-1 overexpressed allele in L1s of the indicated genotypes by RT–qPCR (strains GW1641, GW1658 and GW1646). The mean of two biological replicates is shown together with dots showing data distribution. f, Scheme of the gwIs4 reporter with relevant features. g, Sequence of the baf-1 promoter present in the gwIs4 heterochromatic reporter. The two C/EBP family motifs are in red. h, STRING44 graph showing experimental evidence of protein interaction between the indicated bZIP transcription factors. i, Correlation analysis between ChIP–seq for the indicated histone mark and MRG-1. H3K27ac is shown in red.
