Extended Data Fig. 4: Representative NCP–UV-DDB cryo-EM map segments.
From: DNA damage detection in nucleosomes involves DNA register shifting
a, Different views for the unsharpened local-resolution-filtered map of the NCP6–4PP(−1)–UV-DDB complex. Together with the class averages (Extended Data Fig. 1a), this argues that the density observed is fully accounted for by a single UV-DDB complex engaged with a single damaged nucleosome. b, Representative, sharpened local-resolution-filtered maps of the NCP6–4PP(−1)–UV-DDB complex for which the central DDB1 B-domain features were masked out, showing map segments for the damaged DNA duplex, the DDB2–DNA–histone H3 interface, and H3/H4 position surrounding the damage, and the DDB2 β-hairpin loop involved in damage recognition. c, As in b but with map segments from NCPTHF2(−1)–UV-DDB. d, Sharpened local-resolution-filtered map obtained for NCP6–4PP(−1)–UV-DDB with DDB1 masked out. e, Segments of H2A, H2A, H3, H4, DDB2 β-sheet and DDB2-6–4PP interface excised from the 4.2 Å resolution NCP6–4PP(−1)–DDB2 map shown in d.
