Extended Data Fig. 6: Local intravaginal immunization with HSV-2 is required for the recruitment of memory B cells.
From: Migrant memory B cells secrete luminal antibody in the vagina
C57BL/6 mice were immunized intravaginally or subcutaneously with TK− HSV-2. Five weeks later, mice were challenged with wild-type HSV-2. a, One day after challenge, the number of memory and naive B cells in vaginal tissues was analysed by flow cytometry (naive, n = 4 mice; intravaginal immune, n = 6 mice; subcutaneous immune and wild-type HSV-2, n = 5 mice; intravaginal immune and wild-type HSV-2, n = 5 mice). b, HSV-2-specific IgG2b antibody in serum was measured by ELISA (naive, n = 3 mice; intravaginal immune, n = 3 mice; subcutaneous immune and wild-type HSV-2, n = 5 mice; intravaginal immune and wild-type HSV-2, n = 3 mice). Data are mean ± s.e.m. Data are representative of two independent experiments. Statistical significance was analysed by two-tailed Mann–Whitney U test.
