Extended Data Fig. 7: Antigen-specific recall response is necessary for the recruitment of memory B cells.
From: Migrant memory B cells secrete luminal antibody in the vagina
a, b, C57BL/6 mice immunized with intravaginal TK− HSV-2 five weeks previously were challenged with wild-type HSV-2 or influenza A strain PR8 virus intravaginally (immune, n = 6 mice; immune and wild-type HSV-2, n = 4 mice; immune and PR8, n = 4 mice). a, One day after secondary challenge, the number of memory B cells in vaginal tissues was analysed by flow cytometry. b, Indicated virus titre in vaginal wash was measured one day after secondary challenge. c, C57BL/6 mice immunized intravaginally with TK− HSV-2 five weeks previously were challenged with wild-type HSV-2 or CpG1826 intravaginally. One day after secondary challenge, the number of memory B cells in vaginal tissues was analysed by flow cytometry (immune, n = 5 mice; immune and wild-type HSV-2, n = 6 mice; immune and CpG1826, n = 6 mice). d, e, C57BL/6 mice immunized intravaginally with TK− HSV-2 five weeks previously were intravaginally challenged with active or heat-inactivated (HI) wild-type HSV-2. d, One day after secondary challenge, the number of memory B cells in vaginal tissues was analysed by flow cytometry (immune, n = 5 mice; immune and wild-type HSV-2, n = 7 mice; immune and heat-inactivated HSV-2, n = 14 mice). e, One day after challenge, HSV-2-specific antibodies in vaginal wash were measured by ELISA (immune, n = 19 mice; immune and wild-type HSV-2, n = 10 mice; immune and heat-inactivated HSV-2, n = 9 mice). Sample dilution for ELISA was 1:5. Data are mean ± s.e.m. Data are representative of two independent experiments (a–c) or are pooled from three independent experiments (d, e). Statistical significance was analysed by two-tailed Mann–Whitney U test.
