Extended Data Fig. 2: The vagina is devoid of virus-specific antibodies and tissue-resident B cells even after boosting local inflammation after immunization with HSV-2.
From: Migrant memory B cells secrete luminal antibody in the vagina
C57BL/6 mice were immunized intravaginally with TK− HSV-2. On day 5 after immunization, CpG1826 was injected into the vagina to boost local inflammation of the vaginal mucosa. a, Five weeks later, the number of CD138+CD19+, CD138+CD19− and CD138−CD19+ cells in vaginal tissues was analysed by flow cytometry (naive, n = 4 mice; immune, n = 6 mice; immune and CpG1826, n = 5 mice). b, c, HSV-2-specific antibodies in vaginal wash (b; naive, n = 4 mice; immune, n = 6 mice; immune and CpG1826, n = 5 mice) and in serum (c; naive, n = 3 mice; immune, n = 3 mice; immune and CpG1826, n = 5 mice) were measured by ELISA. Sample dilution for the vaginal wash ELISA was 1:5. Data are mean ± s.e.m. Data are representative of two independent experiments. Statistical significance was analysed by two-tailed Mann–Whitney U test.
