Extended Data Fig. 5: Comparison of the maps in the ligand-binding pocket of hSMO.
From: Cryo-EM structure of oxysterol-bound human Smoothened coupled to a heterotrimeric Gi
a, The extra density within the transmembrane-domain ligand-binding pocket in the hSMO crystal structure (PDB 5L7D). The density is shown in green at the 3σ level, and is indicated by an arrow. b, The density of the ligand in the Gi–hSMO complex. The density is shown in purple mesh at the 5σ level at 3.9 Å, and is indicated by an arrow.
